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Devyser sequence coverage calculator for chimerism


1. Select sequencing system and kit

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2. Select Devyser kit

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Devyser kit / variant Amplicon pool name Samples Minimal coverage per allele Variant Allele Frequency (VAF) Number of reads/read pairs

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Devyser coverage report
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Devyser coverage report

Date:
Sequencing system:
Sequencing kit/flow cell:
Remaining capacity:
PhiX control:
Capacity comment:

Name amplicon pool Devyser kit / variant Number of samples Minimal coverage per variant allele Minimal coverage per amplicon Mean coverage per amplicon Variant Allele Frequency (VAF) Number of reads/read pairs Used kit capacity % of run Volume of amplicon pool [μl]

Instructions
  1. Adjust each library pool to the concentration specified in the Instructions For Use for the specific Devyser kit.
  2. If more than one amplicon pool was prepared, mix the volumes of each library pool as specified in the table above ("Volume of amplicon pool [μl]").
    NOTE For certain combinations of kits and requested VAFs, a large volume ratio for library pooling may be specified, and thus the available volume might not be sufficient for a certain pool. In such cases, we recommend performing multiple parallel purification reactions of the indexed PCR products that can be combined and quantified. Please consult our team at techsupport@devyser.com for more guidance in this matter.
    NOTE When there is capacity remaining in the sequencing kit/flow cell, each amplicon pool will generate proportionally more reads in the sequencing run, which is outlined in the report column "% of run".

For further information please contact techsupport@devyser.com